At 35 years old, Mario De Luna has this physical status:
A product of Chivas youth team, CD Tapatio, de Luna made his professional debut on November 9, 2008 against Puebla F.C. in the 2008 Apertura. At the 2009 Interliga de Luna replaced the injured Jonny Magallón performing brilliantly in the tournament, although he suffered an injury in the final. On September 6, 2009 he scored his first goal in the Primera División de México helping Chivas to a 2-2 draw against Puebla.
On February 21, 2013 sister club Chivas USA announced they had acquired Mario de Luna and teammates Giovani Casillas and Edgar Mejia on loan from CD Guadalajara.
After pushing and verbally abusing a ball boy during a game against the Portland Timbers on May 12, 2013, de Luna earned a yellow card for "Foul and abusive language" directed towards a child.